Технические данные

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Paraformaldehyde-Glutaraldehyde Karnovsky Fixative

Kit contains:

• 2 x 10 ml 16% Paraformaldehyde Solution
• 1 x 10 ml 50% EM Grade Glutaraldehyde
• 1 x 50 ml Sodium Phosphate Buffer 0.2M, pH 7.2

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Technical Data Sheet

Mixture 1 (Original Karnovsky’s Mixture)

• 16% Paraformaldehyde Solution 20 ml
• 50% Glutaraldehyde EM Grade 8 ml
• 0.2M Sodium Phosphate Buffer 25 ml
• Distilled Water 25 ml

Final mixture is 78 ml with 5% Glutaraldehyde, 4% Formaldehyde in 0.064M buffer. This mixture is extremely hypertonic, with an osmolarity of more than 2000m OSM.

Mixture 2

A lower concentration of Glutaraldehyde and Formaldehyde especially used for vascular perfusion.

• 16% Paraformaldehyde Solution 13 ml
• 50% Glutaraldehyde EM Grade 5 ml
• 0.2M Sodium Phosphate Buffer 50 ml
• Distilled Water 32 ml

Final mixture is 2% Paraformaldehyde, 2.5% Glutaraldehyde and 0.1M Buffer. If necessary, the osmolarity can be adjusted by adding sucrose to NaCl. The pH may also need to be adjusted to your desirable point. This mixture is the most effective general fixative for both plant and animal specimens.

Технические характеристики

16% Paraformaldehyde Solution